Bob Hoskins
Role: Eddie Valiant
Christopher Lloyd
Role: Judge Doom
Joanna Cassidy
Role: Dolores
Charles Fleischer
Role: Roger Rabbit / Benny The Cab / Greasy / Psycho (voice)
Kathleen Turner
Role: Jessica Rabbit (voice) (uncredited)
Stubby Kaye
Role: Marvin Acme
Alan Tilvern
Role: R.K. Maroon
Richard LeParmentier
Role: Lt. Santino
Lou Hirsch
Role: Baby Herman (voice)
Betsy Brantley
Role: Jessica's Performance Model
Richard Ridings
Role: Angelo
Edwin Craig
Role: Arthritic Cowboy
Lindsay Holiday
Role: Soldier
Mike Edmonds
Role: Stretch
Morgan Deare
Role: Editor / Gorilla (voice)
Christopher Hollosy
Role: Kid #2
Jean-Paul Sipla
Role: Kid #3
Laura Frances
Role: Blonde Starlet
Joel Cutrara
Role: Forensic #1
Billy J. Mitchell
Role: Forensic #2
Eric B. Sindon
Role: Mailman
Ed Herlihy
Role: Newscaster
James O'Connell
Role: Conductor
Eugene Gutierrez
Role: Teddy Valiant
April Winchell
Role: Mrs. Herman (voice)
Mae Questel
Role: Betty Boop (voice)
Mel Blanc
Role: Daffy Duck / Tweety / Bugs Bunny / Sylvester / Porky Pig (voice)
Tony Anselmo
Role: Donald Duck (voice)
Mary T. Radford
Role: Hippo (voice)
Joe Alaskey
Role: Yosemite Sam / Foghorn Leghorn (voice)
David L. Lander
Role: Smart Ass (voice)
Fred Newman
Role: Stupid (voice)
June Foray
Role: Wheezy / Lena Hyena (voice)
Wayne Allwine
Role: Mickey Mouse (voice)
Russi Taylor
Role: Minnie Mouse / Birds (voice)
Les Perkins
Role: Mr. Toad (voice)
Richard Williams
Role: Droopy (voice)
Pat Buttram
Role: Bullet #1 (voice)
Jim Cummings
Role: Bullet #2 (voice)
Jim Gallant
Role: Bullet #3 (voice)
Frank Sinatra
Role: Singing Sword (voice) (archive sound)
Tony Pope
Role: Goofy / Big Bad Wolf (voice)
Peter Westy
Role: Pinocchio (voice)
Cherry Davis
Role: Woody Woodpecker (voice)
Jack Angel
Role: Additional Voices (voice) (uncredited)
Jeff Arbaugh
Role: Mime (uncredited)
Nancy Cartwright
Role: Dipped Shoe (voice) (uncredited)
Amy Irving
Role: Jessica Rabbit (singing voice) (uncredited)
Mickie McGowan
Role: Mooing Cow / Flying Witch (voice) (uncredited)
Frank Welker
Role: Dumbo (voice) (uncredited)